Effectiveness of Platelet-Rich Fibrin with Decalcified Freeze-Dried Bone Allograft Compared to Decalcified Freeze-Dried Bone Allograft Alone in Mandibular Grade-II Furcation Defects: A Quasi-Experimental Study

ABSTRACT Objective: To assess the effectiveness of platelet-rich fibrin (PRF) with decalcified freeze-dried bone allograft (DFDBA) compared to DFDBA alone in mandibular grade-II furcation defects. Material and Methods: A quasi-experimental study was conducted on nine patients with chronic periodontitis, each having two almost identical mandibular grade II furcation defects. Test sites (left mandibular first molars) were treated with open flap debridement (OFD), DFDBA, and PRF, whereas control sites (right mandibular first molars) received OFD and DFDBA alone. Clinical parameters (plaque index (PI), gingival index (GI), vertical clinical attachment level (VCAL) and horizontal clinical attachment level (HCAL) into the furcation defect) and radiographic measurements (mean alveolar bone defect) were done at baseline and after six months postoperatively. Results: The gain in relative horizontal clinical attachment level (RHCAL) in the test sites was 2.94±0.52 mm compared to 1.33±0.35 mm in control sites (p=0.01). Improvement in mean alveolar bone defect (MABD) (was 1.21±0.5 mm2 at test sites compared to 1.15±0.7 mm2 at control sites) probing pocket depth (PPD), recession, relative vertical attachment level (RVCAL), and percentage of bone fill was found in the test sites compared to control, which statistically insignificant. Conclusion: The test sites had better outcomes than control sites, which was significant for the parameter RHCAL. Therefore, combining the biological benefits of autologous PRF with DFDBA is an efficient and economical treatment modality for the management of mandibular grade II furcation defects.

An in vitro investigation and comparison of the mechanical properties of human amnion and bovine collagen membrane

Introduction: The human amnion membrane is now increasingly used in Guided Tissue Regeneration(GTR). These placental allografts have excellent biologic properties. There is limited information regarding their mechanical properties as compared to other collagen membranes used in GTR. The aim of this study was to test and compare the mechanical properties of human amnion membrane with commercially available bovine collagen. Materials and methods: After measurement of average thickness, three strips of 5×1cm were cut from both amnion and bovine collagen membranes. Mechanical testing was done in a universal testing machine. Test speed was 1mm/min with a load cell of 100N. Tensile strength, Young's modulus and elongation at break were calculated. A suture retention test was also carried out using 3x1 cm size strips. Groups were compared through the Mann-Whitney U test. Results: Average thickness of amnion membrane was 0.46mm and thickness of bovine collagen membrane was 0.37mm.Tensile strength, Young's modulus and elongation at break of amnion membrane were 0.156MPa, 0.645MPa and 17mm; and that of bovine collagen membrane were 2.94MPa, 7.42MPa and 11.00mm, respectively (p = 0.05). Maximum load which can be applied during suturing for amnion membrane was 0.104N and for bovine collagen membrane was 2.07N (p = 0.037). Conclusion: There is no statistically significant difference in terms of tensile strength, Young's modulus and elongation at break between human amnion and bovine collagen membranes.(p=0.05)Membranes can only be considered different on the maximum load which can be applied during suturing.(p=0.037) (AU)